Journal: Journal of Virology
Article Title: Modulation of cytokeratin and cytokine/chemokine expression following influenza virus infection of differentiated human tonsillar epithelial cells
doi: 10.1128/jvi.01460-24
Figure Lengend Snippet: Distribution of CK and lectin staining in HTECs as determined by flow cytometry. ( A ) HTECs were fixed and permeabilized, then stained with monoclonal antibodies specific for CK5, CK14, CK8/18, and CK19 for flow cytometric analysis. Percentages give fraction positive for the indicated CK when gated against matched isotype controls. ( B ) HTECs were stained with MAA I-FITC lectin for avian-adapted α2,3-linked SA receptors or with SNA-BV786 lectin for mammalian-adapted α2,6-linked SA receptors. (B.a) MAA I lectin staining was nearly universal in more than five replicate experiments. (B.b) SNA lectin staining was more limited over the same experiments. (B.c) Dual staining showed that coincident expression of avian with mammalian-adapted SA in HTECs was robust. Gating is based on unstained and fluorescence-minus-one controls as shown in . Inset statistics give means ± SEM of the indicated populations from three replicate experiments.
Article Snippet: Uninfected HTECs were first stained with biotinylated SNA lectin SA and MAA I FITC-conjugated lectin (Vector Laboratories).
Techniques: Staining, Flow Cytometry, Expressing, Fluorescence